N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.
dc.contributor.author | Guan, Ziqiang | |
dc.contributor.author | Delago, Antonia | |
dc.contributor.author | Nußbaum, Phillip | |
dc.contributor.author | Meyer, Benjamin | |
dc.contributor.author | Albers, Sonja-Verena | |
dc.contributor.author | Eichler, Jerry | |
dc.coverage.spatial | England | |
dc.date.accessioned | 2016-12-01T14:13:15Z | |
dc.date.issued | 2016-09 | |
dc.description.abstract | N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation. | |
dc.identifier | ||
dc.identifier.eissn | 1873-3468 | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | Wiley | |
dc.relation.ispartof | FEBS Lett | |
dc.relation.isversionof | 10.1002/1873-3468.12341 | |
dc.subject | N-glycosylation | |
dc.subject | Sulfolobus acidocaldarius | |
dc.subject | archaea | |
dc.subject | dolichol | |
dc.subject | thermoacidophile | |
dc.title | N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier. | |
dc.type | Journal article | |
pubs.author-url | ||
pubs.begin-page | 3168 | |
pubs.end-page | 3178 | |
pubs.issue | 18 | |
pubs.organisational-group | Basic Science Departments | |
pubs.organisational-group | Biochemistry | |
pubs.organisational-group | Duke | |
pubs.organisational-group | School of Medicine | |
pubs.publication-status | Published | |
pubs.volume | 590 |
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