N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.

Guan, Ziqiang

Delago, Antonia

Nußbaum, Phillip

Meyer, Benjamin

Albers, Sonja-Verena

Eichler, Jerry

England

2016-12-01T14:13:15Z

2016-09

N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

http://www.ncbi.nlm.nih.gov/pubmed/27490243

1873-3468

https://hdl.handle.net/10161/13030

eng

Wiley

FEBS Lett

10.1002/1873-3468.12341

N-glycosylation

Sulfolobus acidocaldarius

archaea

dolichol

thermoacidophile

N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier.

Journal article

http://www.ncbi.nlm.nih.gov/pubmed/27490243

3168

3178

18

Basic Science Departments

Biochemistry

Duke

School of Medicine

Published

590