An acoustofluidic trap and transfer approach for organizing a high density single cell array.

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2018-06-22

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Abstract

We demonstrate a hybrid microfluidic system that combines fluidic trapping and acoustic switching to organize an array of single cells at high density. The fluidic trapping step is achieved by balancing the hydrodynamic resistances of three parallel channel segments forming a microfluidic trifurcation, the purpose of which was to capture single cells in a high-density array. Next, the cells were transferred into adjacent larger compartments by generating an array of streaming micro-vortices to move the cells to the desired streamlines in a massively parallel format. This approach can compartmentalize single cells with efficiencies of ≈67% in compartments that have diameters on the order of ∼100 um, which is an appropriate size for single cell proliferation studies and other single cell biochemical measurements.

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10.1039/c8lc00196k

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Ohiri, Korine A, Sean T Kelly, Jeffrey D Motschman, Kevin H Lin, Kris C Wood and Benjamin B Yellen (2018). An acoustofluidic trap and transfer approach for organizing a high density single cell array. Lab on a chip. 10.1039/c8lc00196k Retrieved from https://hdl.handle.net/10161/17203.

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Kris Cameron Wood

Associate Professor of Pharmacology and Cancer Biology

Our laboratory uses genomic and pharmacological approaches to understand how tumor dependencies are shaped by cell intrinsic factors, environmental factors, and drug treatments during the dynamic process of tumor evolution. To learn more, please visit our laboratory website: https://woodlabduke.com/.


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