An acoustofluidic device for the automated separation of platelet-reduced plasma from whole blood.
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2024-01
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Separating plasma from whole blood is an important sample processing technique required for fundamental biomedical research, medical diagnostics, and therapeutic applications. Traditional protocols for plasma isolation require multiple centrifugation steps or multiunit microfluidic processing to sequentially remove large red blood cells (RBCs) and white blood cells (WBCs), followed by the removal of small platelets. Here, we present an acoustofluidic platform capable of efficiently removing RBCs, WBCs, and platelets from whole blood in a single step. By leveraging differences in the acoustic impedances of fluids, our device generates significantly greater forces on suspended particles than conventional microfluidic approaches, enabling the removal of both large blood cells and smaller platelets in a single unit. As a result, undiluted human whole blood can be processed by our device to remove both blood cells and platelets (>90%) at low voltages (25 Vpp). The ability to successfully remove blood cells and platelets from plasma without altering the properties of the proteins and antibodies present creates numerous potential applications for our platform in biomedical research, as well as plasma-based diagnostics and therapeutics. Furthermore, the microfluidic nature of our device offers advantages such as portability, cost efficiency, and the ability to process small-volume samples.
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Ma, Zhehan, Jianping Xia, Neil Upreti, Emeraghi David, Joseph Rufo, Yuyang Gu, Kaichun Yang, Shujie Yang, et al. (2024). An acoustofluidic device for the automated separation of platelet-reduced plasma from whole blood. Microsystems & nanoengineering, 10(1). p. 83. 10.1038/s41378-024-00707-3 Retrieved from https://hdl.handle.net/10161/32120.
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Scholars@Duke

Jianping Xia
Research Interest: Acoustics, acoustic separation, Nano bioparticles manipulation, Exosome

Jean Kwun
Dr. Kwun is currently the Director of Transplant Basic Science Research at the Duke Transplant Center.
Research interests include humoral tolerance to organ transplants in animal model and humans, developing a clinically relevant animal model to study the mechanisms of antibody-mediated rejection (AMR), and establishing a conceptual basis that will translate into therapeutic intervention of AMR.

Eileen Tsai Chambers
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