Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division.

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2013-11-11

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Abstract

Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that Gp0.4 is a direct inhibitor of the E. coli filamenting temperature-sensitive mutant Z division protein. A chemically synthesized Gp0.4 binds to purified filamenting temperature-sensitive mutant Z protein and directly inhibits its assembly in vitro. Consequently, expression of Gp0.4 in vivo is lethal to E. coli and results in bacteria that are morphologically elongated. We further show that this inhibition of cell division by Gp0.4 enhances the bacteriophage's competitive ability. This division inhibition is thus a fascinating example of a strategy in bacteriophages to maximize utilization of their hosts' cell resources.

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10.1073/pnas.1314096110

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Kiro, Ruth, Shahar Molshanski-Mor, Ido Yosef, Sara L Milam, Harold P Erickson and Udi Qimron (2013). Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division. Proceedings of the National Academy of Sciences of the United States of America, 110(48). 10.1073/pnas.1314096110 Retrieved from https://hdl.handle.net/10161/16465.

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Erickson

Harold Paul Erickson

James B. Duke Distinguished Professor Emeritus of Cell Biology

Recent research has been on cytoskeleton (eukaryotes and bacteria); a skirmish to debunk the irisin story; a reinterpretation of proposed multivalent binders of the coronavirus spike protein. I have also published an ebook on "Principles of Protein-Protein Association" suitable for a course module or individual learning.


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