The impact of respiratory gating on improving volume measurement of murine lung tumors in micro-CT imaging
Abstract
<jats:title>ABSTRACT</jats:title><jats:p>Small animal imaging has become essential
in evaluating new cancer therapies as they are translated from the preclinical to
clinical domain. However, preclinical imaging faces unique challenges that emphasize
the gap between mouse and man. One example is the difference in breathing patterns
and breath-holding ability, which can dramatically affect tumor burden assessment
in lung tissue. As part of a co-clinical trial studying immunotherapy and radiotherapy
in sarcomas, we are using micro-CT of the lungs to detect and measure metastases as
a metric of disease progression. To effectively utilize metastatic disease detection
as a metric of progression, we have addressed the impact of respiratory gating during
micro-CT acquisition on improving lung tumor detection and volume quantitation. Accuracy
and precision of lung tumor measurements with and without respiratory gating were
studied by performing experiments with <jats:italic>in vivo</jats:italic> images,
simulations, and a pocket phantom. When performing test-retest studies <jats:italic>in
vivo</jats:italic>, the variance in volume calculations was 5.9% in gated images and
15.8% in non-gated images, compared to 2.9% in post-mortem images. Sensitivity of
detection was examined in images with simulated tumors, demonstrating that reliable
sensitivity (true positive rate (TPR) ≥ 90%) was achievable down to 1.0 mm<jats:sup>3</jats:sup>
lesions with respiratory gating, but was limited to ≥ 8.0 mm<jats:sup>3</jats:sup>
in non-gated images. Finally, a clinically-inspired “pocket phantom” was used during
<jats:italic>in vivo</jats:italic> mouse scanning to aid in refining and assessing
the gating protocols. Application of respiratory gating techniques reduced variance
of repeated volume measurements and significantly improved the accuracy of tumor volume
quantitation <jats:italic>in vivo</jats:italic>.</jats:p>
Type
Journal articlePermalink
https://hdl.handle.net/10161/24255Published Version (Please cite this version)
10.1101/823245Publication Info
Blocker, SJ; Holbrook, MD; Mowery, YM; Sullivan, DC; & Badea, CT (n.d.). The impact of respiratory gating on improving volume measurement of murine lung tumors
in micro-CT imaging. 10.1101/823245. Retrieved from https://hdl.handle.net/10161/24255.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Cristian Tudorel Badea
Professor in Radiology
Our lab's research focus lies primarily in developing novel quantitative imaging systems,
reconstruction algorithms and analysis methods. My major expertise is in preclinical
CT.
Currently, we are particularly interested in developing novel strategies for spectral
CT imaging using nanoparticle-based contrast agents for theranostics (i.e. therapy
and diagnostics).
We are also engaged in developin
Yvonne Marie Mowery
Butler Harris Assistant Professor in Radiation Oncology
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