Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells.

Abstract

Interleukin-17 (IL-17) is a cytokine recently shown to be elevated, along with interferon-γ (IFNγ) and tumor necrosis factor (TNFα), in degenerated and herniated intervertebral disc (IVD) tissues, suggesting a role for these cytokines in intervertebral disc disease. The objective of our study was to investigate the involvement of IL-17 and costimulants IFNγ and TNFα in intervertebral disc pathology. Cells were isolated from anulus fibrosus and nucleus pulposus tissues of patients undergoing surgery for intervertebral disc degeneration or scoliosis. The production of inflammatory mediators, nitric oxide (NOx), prostaglandin E2 (PGE2) and interleukin-6 (IL-6), as well as intercellular adhesion molecule (ICAM-1) expression, were quantified for cultured cells following exposure to IL-17, IFNγ, and TNFα. Intervertebral disc cells exposed to IL-17, IFNγ, or TNFα showed a remarkable increase in inflammatory mediator release and ICAM-1 expression (GLM and ANOVA, p < 0.05). Addition of IFNγ or TNFα to IL-17 demonstrated a synergistic increase in inflammatory mediator release, and a marked increase in ICAM-1 expression. These findings suggest that IVD cells not only respond with a catabolic phenotype to IL-17 and costimulants IFNγ and TNFα, but also express surface ligands with consequent potential to recruit additional lymphocytes and immune cells to the IVD microenvironment. IL-17 may be an important regulator of inflammation in the IVD pathologies.

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Published Version (Please cite this version)

10.1002/jor.21206

Publication Info

Gabr, Mostafa A, Liufang Jing, Antonia R Helbling, S Michael Sinclair, Kyle D Allen, Mohammed F Shamji, William J Richardson, Robert D Fitch, et al. (2011). Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 29(1). pp. 1–7. 10.1002/jor.21206 Retrieved from https://hdl.handle.net/10161/18594.

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Scholars@Duke

Gabr

Mostafa Gabr

Research Associate, Senior

Dr. Gabr's research has specifically focused on the following broad areas: (i) animal model of myelopathy, (ii) participating in clinical trials in spine field.
In the last few years, this research agenda has expanded to include collaborative projects and publications. Dr. Gabr and his colleagues explore benefit of cervical collar following spine fusion, spinal cord injury model, and transforaminal lumbar interbody fusion.
Dr. Gabr is the author of "Interleukin-17 synergizes with IFNIγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells" (Journal of Orthopaedic Research, January 2011), co-author of the "Utility of Cervical Collars Following Cervical Fusion Surgery. Does It Improve Fusion Rates or Outcomes? A Systematic Review" (World Neurosurgery, November 2018).


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