Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells.

Abstract

Interleukin-17 (IL-17) is a cytokine recently shown to be elevated, along with interferon-γ (IFNγ) and tumor necrosis factor (TNFα), in degenerated and herniated intervertebral disc (IVD) tissues, suggesting a role for these cytokines in intervertebral disc disease. The objective of our study was to investigate the involvement of IL-17 and costimulants IFNγ and TNFα in intervertebral disc pathology. Cells were isolated from anulus fibrosus and nucleus pulposus tissues of patients undergoing surgery for intervertebral disc degeneration or scoliosis. The production of inflammatory mediators, nitric oxide (NOx), prostaglandin E2 (PGE2) and interleukin-6 (IL-6), as well as intercellular adhesion molecule (ICAM-1) expression, were quantified for cultured cells following exposure to IL-17, IFNγ, and TNFα. Intervertebral disc cells exposed to IL-17, IFNγ, or TNFα showed a remarkable increase in inflammatory mediator release and ICAM-1 expression (GLM and ANOVA, p < 0.05). Addition of IFNγ or TNFα to IL-17 demonstrated a synergistic increase in inflammatory mediator release, and a marked increase in ICAM-1 expression. These findings suggest that IVD cells not only respond with a catabolic phenotype to IL-17 and costimulants IFNγ and TNFα, but also express surface ligands with consequent potential to recruit additional lymphocytes and immune cells to the IVD microenvironment. IL-17 may be an important regulator of inflammation in the IVD pathologies.

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Published Version (Please cite this version)

10.1002/jor.21206

Publication Info

Gabr, Mostafa A, Liufang Jing, Antonia R Helbling, S Michael Sinclair, Kyle D Allen, Mohammed F Shamji, William J Richardson, Robert D Fitch, et al. (2011). Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 29(1). pp. 1–7. 10.1002/jor.21206 Retrieved from https://hdl.handle.net/10161/18594.

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Fitch

Robert Douglas Fitch

Associate Professor Emeritus of Orthopaedic Surgery

My research interest center around limb lengthening and external fixation. Much is known about the biology of distraction osteogenesis. However, little is known about the effects on the soft tissues with limb lengthening techniques. In a dog model, we are investigating the effects of limb lengthening on muscle function, analizing amount of muscle lengthened per segment of bone lengthening, contractility, and strength. We also hope to begin studies assessing the effects of non steroidal anti-inflammatories on the quality of bone regenerate formed with distraction osteogenesis.


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