Longitudinal intravital imaging of mouse placenta.
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2024-03
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Abstract
Studying placental functions is crucial for understanding pregnancy complications. However, imaging placenta is challenging due to its depth, volume, and motion distortions. In this study, we have developed an implantable placenta window in mice that enables high-resolution photoacoustic and fluorescence imaging of placental development throughout the pregnancy. The placenta window exhibits excellent transparency for light and sound. By combining the placenta window with ultrafast functional photoacoustic microscopy, we were able to investigate the placental development during the entire mouse pregnancy, providing unprecedented spatiotemporal details. Consequently, we examined the acute responses of the placenta to alcohol consumption and cardiac arrest, as well as chronic abnormalities in an inflammation model. We have also observed viral gene delivery at the single-cell level and chemical diffusion through the placenta by using fluorescence imaging. Our results demonstrate that intravital imaging through the placenta window can be a powerful tool for studying placenta functions and understanding the placental origins of adverse pregnancy outcomes.
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Zhu, Xiaoyi, Qiang Huang, Laiming Jiang, Van-Tu Nguyen, Tri Vu, Garth Devlin, Jabbar Shaima, Xiaobei Wang, et al. (2024). Longitudinal intravital imaging of mouse placenta. Science advances, 10(12). p. eadk1278. 10.1126/sciadv.adk1278 Retrieved from https://hdl.handle.net/10161/30645.
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Scholars@Duke
Yubin Kang
Liping Feng
Liping Feng, MD's research has focused on understanding the mechanisms of pregnancy complications associated with placental development. These works are translated then to the clinical care of women through studies dedicated to identify risk factors and novel biomarkers for early prediction and prevention of adverse birth outcomes.
Dr. Feng devotes her entire career to improving pregnancy outcomes through innovative research. Dr. Feng conducts both basic science/laboratory research, as well as participates in clinical studies. Her laboratory has focused on understanding the mechanisms of placenta-originated pregnancy complications such as preeclampsia and still birth, which are important causes of perinatal and neonates’ mortality and morbidity. Currently, she has three lines of investigation focused on the roles of inflammation/infection, cell aging, and environmental exposure in placental development and subsequent pregnancy complications.
In addition, Dr. Feng has established an international collaboration in Global Women’s Health. She has affiliated with the Duke Global Health Institute (DGHI) and participates in a DGHI research. She has an interest in DGHI education, and service or policy initiatives, including mentoring and teaching graduate and professional students on fieldwork and research.
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