Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins.
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2018-06
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Abstract
O-GlcNAc is an intracellular posttranslational modification that governs myriad cell biological processes and is dysregulated in human diseases. Despite this broad pathophysiological significance, the biochemical effects of most O-GlcNAcylation events remain uncharacterized. One prevalent hypothesis is that O-GlcNAc moieties may be recognized by "reader" proteins to effect downstream signaling. However, no general O-GlcNAc readers have been identified, leaving a considerable gap in the field. To elucidate O-GlcNAc signaling mechanisms, we devised a biochemical screen for candidate O-GlcNAc reader proteins. We identified several human proteins, including 14-3-3 isoforms, that bind O-GlcNAc directly and selectively. We demonstrate that 14-3-3 proteins bind O-GlcNAc moieties in human cells, and we present the structures of 14-3-3β/α and γ bound to glycopeptides, providing biophysical insights into O-GlcNAc-mediated protein-protein interactions. Because 14-3-3 proteins also bind to phospho-serine and phospho-threonine, they may integrate information from O-GlcNAc and O-phosphate signaling pathways to regulate numerous physiological functions.
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Toleman, Clifford A, Maria A Schumacher, Seok-Ho Yu, Wenjie Zeng, Nathan J Cox, Timothy J Smith, Erik J Soderblom, Amberlyn M Wands, et al. (2018). Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins. Proceedings of the National Academy of Sciences of the United States of America, 115(23). pp. 5956–5961. 10.1073/pnas.1722437115 Retrieved from https://hdl.handle.net/10161/19689.
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Maria Anne Schumacher
Erik James Soderblom
Director, Proteomics and Metabolomics Core Facility
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