Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor.

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2010-11

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Abstract

Jasmonates are a family of plant hormones that regulate plant growth, development and responses to stress. The F-box protein CORONATINE INSENSITIVE 1 (COI1) mediates jasmonate signalling by promoting hormone-dependent ubiquitylation and degradation of transcriptional repressor JAZ proteins. Despite its importance, the mechanism of jasmonate perception remains unclear. Here we present structural and pharmacological data to show that the true Arabidopsis jasmonate receptor is a complex of both COI1 and JAZ. COI1 contains an open pocket that recognizes the bioactive hormone (3R,7S)-jasmonoyl-l-isoleucine (JA-Ile) with high specificity. High-affinity hormone binding requires a bipartite JAZ degron sequence consisting of a conserved α-helix for COI1 docking and a loop region to trap the hormone in its binding pocket. In addition, we identify a third critical component of the jasmonate co-receptor complex, inositol pentakisphosphate, which interacts with both COI1 and JAZ adjacent to the ligand. Our results unravel the mechanism of jasmonate perception and highlight the ability of F-box proteins to evolve as multi-component signalling hubs.

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Arabidopsis, Inositol Phosphates, Cyclopentanes, Indenes, Plant Growth Regulators, Amino Acids, Isoleucine, Peptide Fragments, F-Box Proteins, Arabidopsis Proteins, Repressor Proteins, Crystallography, X-Ray, Signal Transduction, Binding Sites, Amino Acid Sequence, Protein Structure, Tertiary, Protein Binding, Models, Molecular, Molecular Sequence Data, Oxylipins

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https://hdl.handle.net/10161/21727

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10.1038/nature09430

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Sheard, Laura B, Xu Tan, Haibin Mao, John Withers, Gili Ben-Nissan, Thomas R Hinds, Yuichi Kobayashi, Fong-Fu Hsu, et al. (2010). Jasmonate perception by inositol-phosphate-potentiated COI1-JAZ co-receptor. Nature, 468(7322). pp. 400–405. 10.1038/nature09430 Retrieved from https://hdl.handle.net/10161/21727.

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He

Sheng-Yang He

Benjamin E. Powell Distinguished Professor of Biology

Interested in the fascinating world of plants, microbes or inter-organismal communication and co-evolution? Please contact Prof. Sheng-Yang He (shengyang.he@duke.edu; hes@msu.edu).

Millions of years of co-evolution between plants and microbes have resulted in an intricate web of attack, counter-attack, decoy, and hijacking mechanisms in biology. Moreover, co-evolution between plants and microbes is greatly impacted by ongoing climate change. In our lab, we probe “host-microbe-climate” interactions to answer the following fundamental questions: (1) How do microbial pathogens infect a susceptible host? (2) How do plants select beneficial microbiomes to ensure health? (3) How do climate conditions impact disease and immunity?      

We use contemporary methods to address these questions, including those commonly used in molecular genetics, genomics, biochemistry, cell biology, bioinformatics, microbiology, plant biology, co-evolution and infectious disease biology.    

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