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Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice.

dc.contributor.author Gonzalez, P
dc.contributor.author Luo, X
dc.contributor.author Maurer-Stroh, Sebastian
dc.contributor.author Tanavde, VM
dc.contributor.author Tkatchenko, AV
dc.contributor.author Tkatchenko, TV
dc.contributor.author Vaz, C
dc.contributor.author Young, Terri
dc.contributor.author Zauscher, Stefan
dc.coverage.spatial United States
dc.date.accessioned 2017-08-18T20:12:41Z
dc.date.available 2017-08-18T20:12:41Z
dc.date.issued 2016
dc.identifier https://www.ncbi.nlm.nih.gov/pubmed/27622715
dc.identifier PONE-D-16-12243
dc.identifier.uri http://hdl.handle.net/10161/15319
dc.description.abstract Development of myopia is associated with large-scale changes in ocular tissue gene expression. Although differential expression of coding genes underlying development of myopia has been a subject of intense investigation, the role of non-coding genes such as microRNAs in the development of myopia is largely unknown. In this study, we explored myopia-associated miRNA expression profiles in the retina and sclera of C57Bl/6J mice with experimentally induced myopia using microarray technology. We found a total of 53 differentially expressed miRNAs in the retina and no differences in miRNA expression in the sclera of C57BL/6J mice after 10 days of visual form deprivation, which induced -6.93 ± 2.44 D (p < 0.000001, n = 12) of myopia. We also identified their putative mRNA targets among mRNAs found to be differentially expressed in myopic retina and potential signaling pathways involved in the development of form-deprivation myopia using miRNA-mRNA interaction network analysis. Analysis of myopia-associated signaling pathways revealed that myopic response to visual form deprivation in the retina is regulated by a small number of highly integrated signaling pathways. Our findings highlighted that changes in microRNA expression are involved in the regulation of refractive eye development and predicted how they may be involved in the development of myopia by regulating retinal gene expression.
dc.language eng
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0162541
dc.subject Animals
dc.subject Disease Models, Animal
dc.subject Gene Expression Profiling
dc.subject Gene Regulatory Networks
dc.subject Mice
dc.subject Mice, Inbred C57BL
dc.subject MicroRNAs
dc.subject Myopia
dc.subject Photic Stimulation
dc.subject RNA, Messenger
dc.subject Retina
dc.subject Sclera
dc.subject Sensory Deprivation
dc.subject Signal Transduction
dc.title Large-Scale microRNA Expression Profiling Identifies Putative Retinal miRNA-mRNA Signaling Pathways Underlying Form-Deprivation Myopia in Mice.
dc.type Journal article
pubs.author-url https://www.ncbi.nlm.nih.gov/pubmed/27622715
pubs.begin-page e0162541
pubs.issue 9
pubs.organisational-group Chemistry
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Mechanical Engineering and Materials Science
pubs.organisational-group Ophthalmology
pubs.organisational-group Pathology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published online
pubs.volume 11
dc.identifier.eissn 1932-6203


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